295 Animal Science/Veterinary Medicine
1988 Fitch Avenue
University of Minnesota
St. Paul, MN 55108
Walcheck Lab Web Page
B.S., Montana State University
Ph.D., Montana State University
Post-Doctoral, Boehringer Ingelheim Pharm.
Our research group is examining various aspects of inflammation regulation. We are interested in mechanisms that direct white blood cells (leukocytes) to sites of inflammation and that regulate their effector activities. A key leukocyte involved in the early inflammatory response is the neutrophil, which is the most abundant leukocyte in the blood and typically at sites of acute inflammation. Various inflammatory modulators expressed by neutrophils are regulated by a process referred to as ectodomain shedding. Among other lines of research, my lab is actively involved in understanding the function and regulation of this proteolytic process in order to manipulate inflammation and bolster innate immunity or diminish damaging inflammation.
(For a comprehensive list of Dr. Walcheck's recent publications, refer to PubMed, a service provided by the National Library of Medicine.)
Romee R, Foley B, Lenvik T, Wang Y, Zhang B, Ankarlo D, Luo X, Cooley S, Verneris M, Walcheck B, Miller J. 2013. NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17). Blood. 121:3599
Wiernik A, Foley B, Zhang B, Verneris MR, Warlick E, Ross JA, Weisdorf DJ, Walcheck B, Vallera DA, and Miller JS. 2013. Targeting CD33+ Acute Myeloid Leukemia with a bispecific killer cell engager (BiKE) and ADAM17 inhibition overcomes self inhibitory signals by potent activation through CD16 on NK cells. Clin Cancer Res. 19:3844
Wang Y, Wu J, Newton R, Bahaie NS, Long C, and Walcheck B. 2013. ADAM17 regulates the shedding of CD16b (FcγRIIIb) in human neutrophils. BBA - Mol. Cell Res. 1833:680-685 [Epub ahead of print.]
Long C, Hosseinkhani MR, Wang Y, Sriramarao P, and Walcheck B. 2012. ADAM17 regulates the adhesive properties of circulating neutrophils during E. coli infection. J. Leukoc. Biol. 92: 667
Arndt PG, Strahan B, Wang Y, Long C, Horiuchi K, and Walcheck B. 2011. Leukocyte ADAM17 Regulates Acute Pulmonary Inflammation. PlosOne. 6(5): e19938
Wang Y., Robertson JD, Walcheck B. 2011 Different signaling pathways induce a disintegrin and metalloprotease-17 (ADAM17) in neutrophils during apoptosis and activation. J. Biol. Chem. 286: 38980
Wang Y., Zhang, A., Herrera, AH., Ni, Z and Walcheck, B. 2010. ADAM17 activity and other mechanisms of soluble L-selectin production during death receptor-induced leukocyte apoptosis. J. Immunol. 184:4447
Long C., Wang Y., Herrera, AH., and Walcheck, B. 2010. In vivo role of leukocyte ADAM17 in inflammation induction during E. coli infection. J. Leukoc. Biol. 87:1097
Wang Y., Zhang, A., Herrera, AH., Ni, Z and Walcheck, B. 2009. Induction of ADAM17-mediated ectodomain shedding upon neutrophil apoptosis. Submitted to J. Immunol., currently under revision.
Wang Y., Herrera, AH., Li, Y., and Walcheck, B. 2009. Regulation of mature ADAM17 by redox agents for L-selectin shedding. J. Immunol.182:2449
St Hill, C.A., Farooqui, M., Mitcheltree, G., Gulbahce, H.E., Jessurun, J., Cao, Q., Walcheck, B. 2009. The high affinity selectin glycan ligand C2-O-sLex and mRNA transcripts of the core 2 beta-1,6-N-acetylglusaminyltransferase (C2GnT1) gene are highly expressed in human colorectal adenocarcinomas. BMC Cancer 9:79.
Ni, Z. and Walcheck, B. 2009. Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation. Clinical Immunol. 133:25.
Schaff, U., Mattila, P., Simon, S.I., and Walcheck, B. 2008. Neutrophil adhesion to E-selectin under shear promotes the redistribution and co-clustering of ADAM17 and its proteolytic substrate L-selectin. J. Leukoc. Biol. 83:99
Duchesneau, P., Gallagher, E., Walcheck, B., and Waddell, TK. 2007. Up- regulation of leukocyte CXCR4 expression by sulfatide: An L-selectin- dependent pathway on CD4+ T cells. Eur. J. Immunol. 37:2949
Ni Z, and Walcheck, B. 2007. Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)+ CD4+ T cells. Immunol. Lett. 108:179
Bell, J, Herrera, AH, Li, L., and Walcheck, B. 2007. Role of ADAM17 in the ectodomain shedding of TNF-alpha and its receptors by neutrophils and macrophages. J. Leukoc. Biol. 82:173.
Ni Z, Campbell JJ, Niehans G, Walcheck B. The Monoclonal Antibody CHO-131 Identifies a Subset of Cutaneous Lymphocyte-Associated Antigen T Cells Enriched in P-Selectin-Binding Cells. J. Immunol. 2006 Oct 1;177(7):4742-8.
Li Y, Brazzell J, Herrera A, Walcheck B. ADAM17 deficiency by mature neutrophils has differential effects on L-selectin shedding. Blood. 2006 Oct 1;108(7):2275-9.
Walcheck, B., Herrera, A.H., St. Hill, C.A., Mattila, P., Whitney, A.R., and DeLeo, F.R. 2006. ADAM17 expression and function are sustained during prolonged neutrophil activity. Eur. J. Immunol. 36:968-76.
Mattila, P., Green, C.E., Schaff, U., Simon, S.I., and Walcheck, B. 2005. L-selectin clustering involves altered lipid phase partitioning and is regulated by cytoskeletal anchorage. Amer. J. Physiol. Cell Physiol. 289:323.
St. Hill, C.A., Bullard, K.M. and Walcheck, B. 2005. Expression of the high-affinity selectin glycan ligand C2-O-sLeX by colon carcinoma cells. Cancer Lett. 217:105.
Walcheck, B., Alexander, S.R., St. Hill, C.A., and Matala, E. 2003. ADAM-17-independent shedding of L-selectin. J. Leukoc. Biol. 74:389.
St. Hill, C.A., Shelia R. Alexander, and Bruce Walcheck. 2003. Indirect capture augments leukocyte accumulation on P-selectin in flowing whole blood. J. Leukoc. Biol. 73: 464.
Walcheck, B., Leppanen, A., Cummings, R.D., Knibbs, R.N., Stoolman, L.M., Alexander, S.R., Mattila, P.E., and McEver, R.P. 2002. The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated with sialyl-Lewis x, which is a functional glycan ligand for P-selectin. Blood 99:4064.
Matala, E., Alexander, S.R., Kishimoto T.K., and Walcheck B. 2001. The cytoplasmic domain of L-selectin participates in regulating L-selectin endoproteolysis. J. Immunol. 167:1617.
Alexander, S.R., Kishimoto, T.K., and Walcheck, B. 2000. Effects of selective protein kinase C inhibitors on the proteolytic downregulation of L-selectin from chemoattractant-activated neutrophils. J. Leuk. Biol. 67:415.
Kahn, J., B. Walcheck, G. Migaki, M.A. Jutila, and T.K. Kishimoto. (1998) Calmodulin regulates L-selection adhesion molecule expression and function through a protease-dependent mechansim. Cell 92:809.
Walcheck, B., J. Kahn, J.M. Fisher, B.B. Wang, R.S. Fisk, D.G. Payan, C. Feehan, R. Betageri, K. Darlak, A.F. Spatola, and T.K. Kishimoto. (1996) Neutophil rolling altered by inhibition of L-selectin shedding in vitro. Nature 380:720.
Walcheck, B., K.L. Moore, R.P. McEver, and T.K. Kishimoto. (1996) Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1: A mechanism that amplifies initial leukocyte accumulation on P-selectin in vitro. J. Clin. Invest. 98:1081.