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Kent Reed
 295 Animal Science/Veterinary Medicine 1988 Fitch Avenue University of Minnesota St. Paul, MN 55108 e-mail: reedx054@umn.edu
http://www.tc.umn.edu/~reedx054/
Education B.S., University of Minnesota
M.S., Fort Hays State University
Ph.D., Texas A&M University
Post-Doctoral, University of Rochester, University of Wisconsin—Milwaukee
Research Interests My research is dedicated to the application of molecular and cytogenetic approaches to the study of genomics, immunogenetics and evolution. I have broad training in genetics, evolutionary biology and molecular biology with 12 years of experience working in avian genomics and poultry science. My laboratory has led the effort to develop genetic resources for the turkey, culminating in a medium density genetic linkage map in support of the recent assembly and analysis of a draft genome sequence. We are part of an ongoing effort to complete the sequence of the turkey genome using next-generation sequencing. Additional research is directed towards the application of these genomic resources for collaborative functional investigations in muscle physiology, immunology and toxicology. Projects have included a comprehensive approach to investigation of muscle function and development as it relates to meat production and an examination of gene expression associated with cardiomyopathy (Round Heart Disease). A second project focuses on the structure and evolution of the turkey major histocompatibility complex (MHC). The main objectives of this project are to sequence the turkey MHC; identify important haplotypes; develop and trial assays for genetic testing of commercial and experimental populations; and explore expression of key genes. Our efforts have made the turkey one of the better characterized avian species at the MHC. We are currently using RNA-seq approaches to better understand the metabolic and immunological responses of turkey to probiotic and aflatoxin exposure. These studies are aimed at mitigating the effects of mycotoxin contaminants in feed. Selected Publications
(For a comprehensive list of Dr. Reed's recent publications, refer to PubMed, a service provided by the National Library of Medicine.) Reed KM. 2009. Turkey genetic mapping. Chapter 6, In: Genome Mapping and Genomics in Domestic Animal (Genome Mapping and Genomics in Animals Series, Volume 3), Pp. 143-163. Springer-Verlag, Berlin. Chaves LD, Harry DE and Reed KM. 2009. Genome-wide genetic diversity of "Nici", the DNA source for the CHORI-260 turkey BAC library and candidate for whole genome sequencing. Animal Genetics, 40:348-352. Rawal S, Mendoza KM, Reed KM, and Coulombe RA, Jr. 2009. Structure, genetic mapping and function of the Cytochrome P450 3A37 gene in the turkey (Meleagris gallopavo). Cytogenetic and Genome Research, 125:125:67-73. Phillips RB, DeKoning JJ, Ventura AB, Nichols KM, Drew RE, Chaves LD, Reed KM, Felip A, and Thorgaard GH. 2009. Recombination is suppressed over a large region of the rainbow trout Y chromosome. Animal Genetics, 40:925-932. Chaves LD, Kreuth SB, and Reed KM. 2009. Defining the turkey MHC: sequence and genes of the B-locus. J Immunology, 183:6530-6537. Epub October 28 2009. Kim JE, Bauer MM, Mendoza KM, Reed KM, and, Coulombe RA, Jr. 2010. Comparative genomics identifies new alpha class genes within the avian glutathione S-transferase gene cluster. Gene, 452:45-53 Epub 10 November 2009. Reed KM, Bauer MM, Mendoza KM, and Armien AG. 2010. A candidate gene for choanal atresia in alpaca. Genome, 53:224-230. Chaves LD, Faile GM, Kreuth SB, Hendrickson JA, and Reed KM. 2010 Haplotype variation, recombination, and gene conversion within the MHC-B of the turkey. Immunogenetics, 62:465-477. Epub 12 May 2010. Dalloul RA, Long JA, Zimin AV, Aslam L, Beal K, Ann Blomberg L, Bouffard P, Burt DW, Crasta O, Crooijmans RP, Cooper K, Coulombe RA, De S, Delany ME, Dodgson JB, Dong JJ, Evans C, Frederickson KM, Flicek P, Florea L, Folkerts O, Groenen MA, Harkins TT, Herrero J, Hoffmann S, Megens HJ, Jiang A, de Jong P, Kaiser P, Kim H, Kim KW, Kim S, Langenberger D, Lee MK, Lee T, Mane S, Marcais G, Marz M, McElroy AP, Modise T, Nefedov M, Notredame C, Paton IR, Payne WS, Pertea G, Prickett D, Puiu D, Qioa D, Raineri E, Ruffier M, Salzberg SL, Schatz MC, Scheuring C, Schmidt CJ, Schroeder S, Searle SM, Smith EJ, Smith J, Sonstegard TS, Stadler PF, Tafer H, Tu ZJ, Van Tassell CP, Vilella AJ, Williams KP, Yorke JA, Zhang L, Zhang HB, Zhang X, Zhang Y, and Reed KM. 2010. Multi-platform next generation sequencing of the domestic turkey (Meleagris gallopavo): Genome assembly and analysis. PLoS Biol 8(9): e1000475. doi:10.1371/journal.pbio.1000475. Sporer KRB, Chiang W, Tempelman RJ, Ernst CW, Reed KM, Velleman SG, Strasburg GM. 2010. Characterization of a 6K oligonucleotide turkey skeletal muscle microarray to profile gene expression changes during muscle development. Animal Genetics, Epub 26 May 2010 [ahead of print]. Chaves LD, Kreuth SB, Bauer MM, and Reed KM. 2010. Sequence of a turkey BAC clone identifies MHC class III orthologs and supports ancient origins of immunological gene clusters. Cytogenetic and Genome Research, Epub 24 August 2010 [ahead of print]. Current Funding "Reference genome sequence for the turkey, Meleagris gallopavo," AFRI, USDA. "The turkey MHC: Sequence, haplotype structure and gene expression," CSREES, USDA. "RNA-Seq in horse and turkey transcriptomics." MAES. Current Students Miranda Bauer, PhD candidate, Genetics and Genomics of Turkey B-G genes Melissa Monson, PhD Student, Gene expression in the turkey MHC.
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