When
July 9-13
Where
College of Veterinary Medicine, Veterinary Science Building
Fee
$1125 through July 1, $1225 after July 1
secure online registration
Lecture Schedule: 8:00 - 10:30 a.m.
Date Topic
July 9 Organization and structure of biological information
July 10 DNA synthesis, replication and PCR
July 11 Recombinant DNA basics – from life to lab
July 12 Gene expression
July 13 Real-time PCR, DNA microarrays, and Bioinformatic analyses
Laboratory Schedule: 10:30 a.m. - 5:00 p.m.
Date Topic
July 9 Good lab practice. Isolation and analysis of DNA.
July 10 DNA synthesis by PCR. Restriction digest of DNA.
July 11 DNA sequencing. Recombining DNA.
July 12 RNA purification. Bacterial transformation. Real-time PCR.
July 13 Bioinformatics. Genetic analysis.
Monday Laboratory
Lab 1A: Plasmid Extraction (Animal)
I: Extraction of plasmids from culture (Qiagen kit)
II: Agarose gel to determine concentration
Lab 1B: Genomic DNA Extraction (Plant)
I: Extraction of genomic DNA from powdered oat tissue
II: Agarose gel to visualize difference between genomic DNA and plasmidI
III: Set up PCR reaction
Lab 2A: Sequence sample preparation (Animal)
I: Set-up of samples of plasmid for sequencing
Lab 2B: Genomic DNA extraction (Plant) for PCR (continued from lab 1Bb)
I: Agarose gel of PCR
Lab 2C: Sensitivity of detection of transgenic material (Plant)
I: Dilution of transgenic soybean with nontrangenic
II: PCR set-up using primers specific to transgene bar (Qiagen Master Mix)
Lab 2D: Analysis of pGLO and pSK constructs (Bacteria)
I: Restriction enzyme of pGLO and pSK constructs (EcoRI and XbaI)
II: Agarose gel to visualize digestion of plasmids
Wednesday Laboratory
Lab 3A: Tour of sequencing facility AGAC - 10:30am to 11:15pm (Animal)
Lab 3B: Sensitivity issue association with detecting transgenic material (Plant)
I: Agarose gel to visualize PCR from soybean samples
Lab 3C: Analysis of pGLO and pSK construct (Bacteria)
I: Digestion clean up - Qiagen PCR Clean up kit
II: Ligation of pGLO and pSK
Thursday Laboratory
Lab 4A: Expression analysis of transgene in soybean material (Plant)
I: Extraction of total RNA from soybean material (Qiagen kit)
II: Set-up of reverse-transcriptase PCR (Qiagen one-step)
Lab 4B: Transformation of pGLO, pSK and pGLO+pSK constructs (Bacteria)
I: Chemical competent cells multiply above plasmids (Invitrogen TOP 10)
Lab 4C: Real-time PCR
Friday Laboratory
Lab 5A: Bioinformatics analysis of sequence material (Animal)
I: Computer analysis
Lab 5B: Expression analysis of transgene in soybean material (Plant)
I: Agarose gel of RT-PCR
Lab 5C: Analysis of pGLO, pSK, and pGLO+pSK constructs (Bacteria)