295H Animal Science/ Veterinary Medicine
1988 Fitch Avenue
University of Minnesota
St. Paul, MN 55108
B.S. and M.A., University of California, Los Angeles (UCLA)
Ph.D., University of North Carolina, Chapel Hill (UNC)
Post-doctoral, University of California, San Francisco (UCSF)
Molecular mechanisms of Lassa fever virus replication and host immune suppression: Lassa fever virus infection can lead to severe and sometime lethal hemorrhagic fever diseases in humans, for which there is no vaccine or effective drug. Studies of Lassa (LASV) and other pathogenic arenaviruses have been hindered by the strict requirement for BSL-4 containment to work with these select agents. We have recently developed an infectious molecular clone for a prototypic BSL2 arenavirus (i.e., Pichinde virus - PICV) as well as a safe and convenient mini-replicon system for LASV. We have also developed a safe and useful small animal model for Lassa fever by infecting guinea pigs with PICV. These novel systems have afforded us with unique resources and unprecedented opportunities to investigate in details the processes of viral genome replication/transcription and virulence. We are also interested in characterizing a novel functional mechanism of the Lassa viral nucleoprotein in mediating host innate immune suppression (Qi, et al., 2010, Nature, 468: 779-783). These efforts may lead the development of effective therapeutics against this deadly viral pathogen that can claim up to 5,000 deaths annually in the endemic areas of West Africa and is a constant bio-threat to our national security.
Roles of telomere-telomerase biology in human blood disorders and cancers: Patients with a serious form of blood disorder known as acquired aplastic anemia (AA) have very low blood counts due to marrow failure syndromes. We have recently shown that naturally occurring mutations in the telomere maintenance genes can predispose some of the patients to the development of AA (Yamaguchi, et al., 2005, New Engl J of Med; 2010, British J Haematol). The exact mechanisms of how these germ-line mutations lead to limited marrow stem-cell reserve and renewal capacity have not been characterized in details. We aim to investigate the molecular mechanisms by which telomere dysfunction can lead to ineffective hematopoiesis and increased cancer risk in patients with bone-marrow failure syndromes.
(For a comprehensive list of Dr. Ly's recent publications, refer to PubMed, a service provided by the National Library of Medicine.)
Comparative analysis of disease pathogenesis and molecular mechanisms of New World and Old World arenavirus infections. McLay LJ, Liang Y, Ly H. J Gen Virol. 2013 Sep 25. [Epub ahead of print]
Structures of arenaviral nucleoproteins with triphosphate dsRNA reveal a unique mechanism of immune suppression. Jiang X, Huang Q, Wang W, Dong H, Ly H*, Liang Y*, Dong C*. J Biol Chem. 2013 Jun 7;288(23):16949-59. (*corresponding authors)
Identification of virulence determinants within the L genomic segment of the pichinde arenavirus. McLay L, Lan S, Ansari A, Liang Y, Ly H. J Virol. 2013 Jun;87(12):6635-43.
Targeting virulence mechanisms for the prevention and therapy of arenaviral hemorrhagic fever. McLay L, Ansari A, Liang Y, Ly H. Antiviral Res. 2013 Feb;97(2):81-92.
Characterization of virulence-associated determinants in the envelope glycoprotein of Pichinde virus. Kumar N, Wang J, Lan S, Danzy S, McLay Schelde L, Seladi-Schulman J, Ly H*, Liang Y*. Virology. 2012 Nov 10;433(1):97-103. (*corresponding authors)
Biological roles and functional mechanisms of arenavirus Z protein in viral replication. Wang J, Danzy S, Kumar N, Ly H, Liang Y. J Virol. 2012 Sep;86(18):9794-801.
Telomere dynamics in induced pluripotent stem cells: Potentials for human disease modeling. Ly H. World J Stem Cells. 2011 Oct 26;3(10):89-95.
Cap binding and immune evasion revealed by Lassa nucleoprotein structure. Qi X, Lan S, Wang W, Schelde LM, Dong H, Wallat GD, Ly H*, Liang Y*, Dong C*. Nature. 2010 Dec 9;468(7325):779-83. (*corresponding authors)
Identification of TINF2 gene mutations in adult Japanese patients with acquired bone marrow failure syndromes. Yamaguchi H, Inokuchi K, Takeuchi J, Tamai H, Mitamura Y, Kosaka F, Ly H, Dan K. Br J Haematol. 2010 Sep;150(6):725-7.
Molecular determinants of Pichinde virus infection of guinea pigs--a small animal model system for arenaviral hemorrhagic fevers. Liang Y, Lan S, Ly H. Ann N Y Acad Sci. 2009 Sep;1171 Suppl 1:E65-74.
"Novel mechanism of immune evasion by arenaviral nucleoproteins", NIH/NIAID, R01 AI093580-01, 2012-2017, Ly (PI)
"Cap snatching mechanism of arenaviruses", NIH/NIAID, R56 AI091805-01, 2011-2013, Ly (PI)
Honors and Awards
2006-2011 American Cancer Society Scholar Award
2006-2008 Established Researcher Award, AA&MDS Int’l Foundation
2008 Inaugural One-in-One-Hundred Mentor Award (Emory University)
2003-2006 Leukemia & Lymphoma Society of America Special Fellowship