Murtaugh Lab Protocols

Real-time RT PCR Protocols

• Real-time PCR Protocol 7500 SYBR Green Assay
• Real-time PCR Analysis: Pfaffl Method
• Real-time PCR Efficiency Calculation using LinReg
• PRRSV Real-time PCR Protocol 7500

RNA Isolation Protocols For Real-Time PCR

• RNA Isolation from Biological Fluids
• RNA Isolation of Standard Curve
• RNA Isolation from Tissue
• RNA Isolation from Tonsil Scrapings

Microarray Protocols

• Array Probe Preparation and Hybridization
• Quantarray Software Procedure
• ScanArray Procedure
• Using GeneSpring

ELISA Protocols

Protein Protocols

Other Protocols

• Using the Ussing Chamber