Submitting Cheek Swabs
Genetic Testing Protocol
The Veterinary Diagnostic Laboratory encourages the submission of samples thru your veterinarian, and the reporting of results to them. Direct involvement of your veterinarian allows for optimal management of this genetic disease as well as other concurrent medical conditions your dog may have that will affect its management. If owners would also like a copy of the report sent directly to them for their records, please include the owner's email address in the Result Reporting section of the submission form (EIC Form, LPN1 Form, AM-PN Form). Please complete the form online and print it out. This greatly decreases the chance for typos in the report, and helps keep administrative costs down. Complete the form with all the required information on the dog to be tested. If more than one dog is being tested at the same time, complete a form for each dog even if they are from the same litter.
For VPI (verified permanent identification) level registration with OFA, a permanent ID must be verified and signed off by the attending veterinarian on the submission form.
Fees for these tests can be found on the guide and fees page. If you are enclosing payment, please indicate so on the submission form and place a check or money order (made payable to "Regents of the University of Minnesota") in a ziploc bag. For further information on reporting and billing policies, including payment options, please click here.
Types of Swabs Accepted
The VDL does not provide cheek swab collection kits. You may purchase appropriate cheek swabs (also called Sterile Applicators) at certain pharmacies, especially specialty pharmacies and medical supply stores that carry home health care products. You may also order them online from a variety of vendors, such as:
Regardless of how they are obtained, all Cheek Swabs used for EIC, LPN1 or AM-PN testing MUST:
- Be STERILE - cotton, polyester or foam tipped applicators.
- Be individually wrapped or have TWO sterile swabs per package, and the package must be intact and unopened.
- Have a plastic or wooden handle that is at least 6 inches long. Shorter (3 inch) swabs are NOT acceptable. Q-Tip type all purpose swabs are NOT acceptable, but Q-Tip brand cotton applicators are okay.
- Be DRY. Swabs with transport medium are NOT acceptable.
The VDL reserves the right to reject unacceptable swabs and packaging of samples.
Preparation Prior to Collection
Please note: cheek swabs are NOT ACCEPTED for unweaned puppies. Puppies MUST be weaned for at least 24 hours before swabbing.
The dog to be sampled should not eat or drink for at least one hour prior to having the sample collected. This is to reduce the chance of contamination.
The dog should be isolated from other dogs, toys, rawhides, and other possible sources of oral contamination for at least one hour prior to sampling. This is to reduce the chance of dog to dog contamination.
Just prior to sampling, check the dog’s mouth to see that no food or other material is obviously present. If there is, clean/rinse the dog’s mouth to remove it.
The person taking the samples should avoid touching the inside of the dog’s mouth. Disposable latex gloves will help reduce contamination by the handler.
If more than one dog is being sampled, the person doing the collections must wash hands or change gloves between collections.
Never allow the sterile swab to touch anything except the inside of the dog’s mouth or the inside of the package that it came in. Especially never allow contact between swabs from different dogs.
Prepare a clean surface on which to place the opened swab package while you are swabbing the dog.
1. Use TWO swabs per dog.
2. Label the swab packets with the dog's call name, the owner's last name, permanent identification or registration number (if applicable) and collection date.
3. Label a standard paper (letter size) envelope with the same information as in #2.
4. Open the swab package at the handle end (NOT the collection tip end) and carefully peel the package away to within about an inch of the end. Remove the first swab. Do not touch the collection tip of the swab.
5. Do not allow the tip of the swab to touch anything other than the inside of the dog's mouth and the inside of the packaging.
6. While holding the handle end of the swab, insert the tip along the inside of the cheek. Rotate the collection tip along the inside of the cheek for 20 seconds. Push the outside of the dog's cheek while rotating the swab and firmly press the swab between the gum and cheek to enhance cell collection. The VDL will accept ONE free re-submission of appropriate swab types that give an indeterminate result due to insufficient cell collection.
7. Hold the swab while it air dries for 10 seconds. Do NOT blow on the swab.
8. Reinsert the tip of the swab into the closed end of the package and remove the second swab. If your swabs came in plastic containers, put the swab directly into the labeled paper envelope you have prepared. DO NOT use the plastic container.
9. Repeat the above steps on the other cheek with the remaining swab (i.e. 2 swabs/dog).
10. Place the packaged swabs in the labeled paper envelope (NOT a plastic bag). Do NOT put the submission form in the envelope with the swabs.
11. Send the sample and completed submission form following the packaging instructions below.
Packing and Shipping
A major concern in using cheek swabs is the possibility of microbial growth on the swab after the sample has been collected. This is particularly a problem during hot and humid times of the year. To reduce the chance for contamination, do not store the swabs (used or unused) in sealed plastic bags. Do not freeze them or store them in the refrigerator where condensation can more easily occur. Ice packs should NOT be used for shipping cheek swab samples. Be prepared to ship the cheek swab sample as soon as possible after it is collected.
By following the instructions you will have placed the air-dried swabs in a labeled, clean paper envelope. Do NOT put the submission form in the envelope with the swabs. Place this labeled envelope and the submission form (and payment by check or money order if applicable) into a sturdy mailing envelope. Write “Do Not Bend” on the outside. Send this package to:
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave
St. Paul, MN 55108
Phone: (612) 625-8787
If you are shipping samples from outside the United States, please review these USDA Guidelines for Importation.
Cheek Swab testing protocol adapted from OptiGen, LLC
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