VDL
What's Inside


College Links

 About

 Directory

 Employment

 Map/Directions

 Volunteer

Search

 

 

Make a Gift box


  Home > Submission Guidelines > Submission for Electron Microscopy
 

Submission for Electron Microscopy

Pathogen Identification

Clinical cases

  • Sample type (table) and quantity:
  • Secretion, exudates, lavage, fluid, and feces: >0.3 ml
  • Papule and tissue: >0.3mm (biopsy)
  • Sample collection:
  • Sample must be collected in any available sterile vial and submitted fresh or refrigerated at 4 degrees C.
  • Sample must be correctly identified with case number, animal species, type of sample, date of collection, submitter contact information.

Cultures isolate (Please contact Dr. Aníbal G. Armién):

  • Known agents (samples with control cell line for negative stain)
  • Unknown agents (samples with control cell line for negative stain and isolate embedding)

Tissue Ultrastructure

  • Tissues fixed as soon as possible in one of the following EM fixatives (optimal method):
  • 2.5% Glutaraldehyde, 0.1M Sodium Cacodylate buffer
  • 2.0% Paraformaldehyde/ 0.5% Glutaraldehyde, 0.1M Sodium Cacodylate buffer
  • Karnovsky’s Solution

Biopsy or any tissue collected from a fresh animal carcass should be placed in EM fixative, ideally within a period of 6 hours after death.

  • Tissue fixed in 10% Neutral Buffered Formalin (NBF)

    Biopsy or any tissue collected from a fresh animal carcass should be placed in 10% NBF, ideally within a period of 3 hours after death. No tissue size is required.

  • Paraffin-embedded tissue

    Tissues fixed in NBF from biopsy or a fresh animal carcass, ideally collected within a period of 1 hour after death.

Sample Submission  

Please submit samples using our submission form, with full information:

  • Disease history
  • Pathology report and diagnosis
  • H & E slide (if available)

Collaborations

Collaboration and Co-authorship: The Diagnostic Ultrastructural Pathology Service offers a full diagnostic service; selected staff members of the Ultrastructural Pathology Section are requested to be considered as co-authors when data generated in our service are being published.

Materials and Methods: Please contact us for manuscript preparation (methods and materials, results sections – e.g., description, images).


Notice of Privacy Practices