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Bacteriology
Effective February 28, 2013
| | | Note: A $10 accession fee will be applied to all submissions. All fees are in US $. | Click the name of the test or procedure for detailed information. Tests marked with an asterisk (*) will redirect to the Bacterial PCR page. Tests marked with a double asterisk (**) have the following disclaimer: Poultry Salmonella Testing is for BAH/NPIP approved programs only. | - Acid Fast Stain
- Actinobacillus pleuropneumoniae - APP (PCR)*
- Actinobacillus pleuropneumoniae - APP (PCR), Serotypes 3, 6 and 8*
- Actinobacillus pleuropneumoniae/Actinobacillus suis Multiplex - APP/A. suis (PCR)*
- Actinobacillus pleuropneumoniae - APP Serotyping (PCR)*
- Actinobacillus suis (PCR)*
- Aerobic Culture (Including Urine Culture)
- Anaerobic Culture
- Aquaculture Bacterial Inspection
- Bacterial Identification by DNA Sequencing (16S rRNA gene)*
- Bacterial Isolate Send Out
- Biotyping, Pasteurella multocida
- Blood Culture, Aerobic
- Blood Culture, Anaerobic
- Brachyspira spp. Culture
- Brachyspira hyodysenteriae and Brachyspira pilosicoli Multiplex PCR*
- Campylobacter Culture, Bovine Reproduction
- Campylobacter Culture, Small Animal
- Chlamydia (Chlamydophila) Antigen Capture (ELISA)
- Chlamydia (Chlamydophila) Isolation (Cell Culture)
- Clostridium difficile (ELISA)
- Clostridium perfringens Toxin Gene Typing (PCR)*
- Dark Field Microscopy for Brachyspira/Spirochetes
- Dark Field Microscopy for Tritrichomona
- Environmental Culture
- Erysipelothrix rhusiopathiae (PCR)*
- Escherichia coli Virulence Factors (PCR)*
- Fluorescent Antibody, Clostridia spp. (chauvoei, novyi, sordellii, septicum)
- Fungal Culture
- Fungal Stain (Fluorescent and KOH)
- Genotype Analysis, Bacterial (BioNumerics)*
- Giemsa Stain
- Gimenez Stain
- Gram Stain
- Haemophilus parasuis (PCR)*
- Isolate Identification
- Lawsonia intracellularis (PCR), Equine
- Lawsonia intracellularis (PCR), Porcine
- Leptospira sp. (PCR)*
- Listeria Culture
- Mycobacterium paratuberculosis - Johne's Culture
- Mycobacterium paratuberculosis - Johne's (PCR)
- Mycoplasma gallisepticum (PCR)*
- Mycoplasma hyopneumoniae (PCR)*
- Mycoplasma hyopneumoniae Culture in Broth
- Mycoplasma hyopneumoniae Genetic Analysis (P146 Adhesin sequencing)*
- Mycoplasma hyorhinis (PCR)*
- Mycoplasma hyosynoviae (PCR)*
- Mycoplasma iowae (PCR)*
- Mycoplasma meleagridis (PCR)*
- Mycoplasma Speciation
- Mycoplasma synoviae (PCR)*
- Mycoplasma spp. Culture
- Pasteurella multocida Toxin Testing (PCR)*
- Salmonella cholerasuis Argus-Vaccine Identification*
- Salmonella enterica Real-time PCR (TaqMan®)*
- Salmonella spp. Culture
- Salmonella spp. Culture - Primary Culture**
- Salmonella spp. Restart Culture**
- Salmonella spp. Screen (Delayed Enrichment)
- Salmonella spp. Screen (Delayed Enrichment) - Secondary Culture**
- Salmonella spp. Screen (Eggs APHIS "Egg Sampling Method")
- Salmonella spp. Serogrouping
- Salmonella spp. Serogrouping**
- Salmonella spp. Serotyping
- Salmonella spp. Serotyping**
- Serotyping (Actinobacillus pleuropneumoniae, Streptococcus suis, Haemophilus parasuis)
- Streptococcus suis (PCR)*
- Streptococcus suis Serotyping (PCR), Serotypes 1,2,7 and 9*
- Susceptibility Testing/MIC
- Tritrichomonas spp. Culture
- Ureaplasma spp. Culture
- Urolith Culture
- Water Culture
(includes counts on total Gram negative, Gram negative lactose fermenters (coliforms), Gram negative non-lactose fermenters, and Pseudomonas aeruginosa) - Yersinia Culture
| Acid Fast Stain | | Fee: 10.25 | Specimen: 1) affected tissue 2) smear or slide 3) swab | Shipping Container: 1) sterile container 2) slide folder 3) culturette | Coolant: 1,3) frozen gel packs 2) none | Days Tested: M-F | Turnaround Time: 1 bus. day | | Comments: | Top of Page | Aerobic Culture (Including Urine Culture) | | Fee: 21.50 | Specimen: 1) swab 2) affected tissue | Shipping Container: 1) culturette 2) sterile container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 2-5 bus. days | | Comments: Samples should be kept cool and moist in transit. | Top of Page | Anaerobic Culture | | Fee: 23.25 | Specimen: 1) swab 2) affected tissue | Shipping Container: 1) anaerobic transport media 2) sterile container | Coolant: 1) none 2) frozen gel packs | Days Tested: M-F | Turnaround Time: 2-5 bus. days | | Comments: Ship overnight. | Top of Page | Aquaculture Bacterial Inspection | Fee: 94.50 per lot (up to 60 fish) | Specimen: live fish | Shipping Container: leakproof container | Coolant: none | Days Tested: M-W | Turnaround Time: 10 bus. days | | Comments: See the Aquaculture/Fisheries Submission Form for details on tests and pathogens. Does not include Renibacterium salmoninarum IFAT. | Top of Page Top of Page | Biotyping, Pasteurella multocida | | Fee: 20.00 | Specimen: pure culture | Shipping Container: agar plate or slant | Coolant: none | Days Tested: M-F | Turnaround Time: 7 bus. days | | Comments: Prevent agar from freezing. | Top of Page | Blood Culture, Aerobic | | Fee: 26.25 | Specimen: 5 ml blood | Shipping Container: 100 ml blood culture bottles | Coolant: none | Days Tested: M-F | Turnaround Time: 2-8 bus. days | | Comments: | Top of Page | Blood Culture, Anaerobic | | Fee: 29.00 | Specimen: 5 ml blood | Shipping Container: 100 ml blood culture bottles | Coolant: none | Days Tested: M-F | Turnaround Time: 3-10 bus. days | | Comments: | Top of Page | Brachyspira spp. Culture | | Fee: 26.25 | Specimen: 1) fresh colon section 2) fresh feces | Shipping Container: 1,2) sterile container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 10-14 bus. days | | Comments: Ship overnight or deliver the same day. | Top of Page | Campylobacter Culture, Bovine Reproduction | | Fee: 34.75 | Specimen: 1) aborted fetus 2) prepucial flush 3) cervical mucous | Shipping Container: 1) sterile container 2,3) Campy transport media | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 6-14 bus. days | | Comments: Transport media is supplied by the laboratory. | Top of Page | Campylobacter Culture, Small Animal | | Fee: 34.75 | Specimen: 1) feces 2) intestine (tied loop) | Shipping Container: 1,2) sterile container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 6-14 bus. days | | Comments: Use anaerobic transport media if delayed more than 24 hrs. | Top of Page | Chlamydia (Chlamydophila) Antigen Capture (ELISA) | | Fee: 30.25 | Specimen: swabs | Shipping Container: plastic tube | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 2-3 bus. days | | Comments: | Top of Page | Chlamydia (Chlamydophila) Isolation (Cell Culture) | | Fee: 37.25 | Specimen: feces in Chlamydia transport media | Shipping Container: sealed container | Coolant: frozen gel packs | Days Tested: Th | Turnaround Time: 2-3 weeks | | Comments: Contact lab for transport media. | Top of Page | Clostridium difficile (ELISA) | | Fee: 16.50 | Specimen: 1) 1 gm intestinal contents, or 2) 1 gm of feces | Shipping Container: 1,2) plastic bag or tube | Coolant: frozen gel packs | Days Tested: once weekly | Turnaround Time: 2-7 bus. days | | Comments: Tests run when sufficient number of samples are available. | Top of Page | Dark Field Microscopy for Brachyspira/Spirochetes | | Fee: 8.75 | Specimen: 1) spiral colon 2) fresh feces | Shipping Container: 1,2) sterile container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 1 bus. day | | Comments: Ship overnight or deliver the same bus. day. | Top of Page | Dark Field Microscopy for Tritrichomona | | Fee: 9.50 | Specimen: 1) prepucial flush 2) cervical mucus | Shipping Container: 1,2) sterile container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 1 bus. day | | Comments: Ship overnight or deliver the same bus. day. | Top of Page | Environmental Culture | | Fee: 32.75 | Specimen: swab | Shipping Container: culturette | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 2-5 bus. days | | Comments: Samples should be kept cool and moist in transit. | Top of Page | Fluorescent Antibody, Clostridia spp. (chauvoei, novyi, sordellii, septicum) | | Fee: 15.25 | Specimen: 1) affected tissue 2) 5 thin smears | Shipping Container: 1) sterile container 2) slide folder | Coolant: 1) frozen gel packs 2) none | Days Tested: M-F | Turnaround Time: 1 bus. day | | Comments: Fix slides in acetone for 5 minutes. | Top of Page | Fungal Culture | | Fee: 21.75 | Specimen: 1) affected tissue 2) hair or scraping 3) swab | Shipping Container: 1) sterile container 2) envelope 3) culturette | Coolant: 1,3) frozen gel packs 2) none | Days Tested: M-F | Turnaround Time: 4-6 weeks | | Comments: | Top of Page | Fungal Stain (Fluorescent and KOH) | | Fee: 14.75 | Specimen: 1) affected tissue 2) smear or slide 3) swab 4) hair sample | Shipping Container: 1) sterile container 2) slide folder 3) culturette 4) envelope | Coolant: 1,3) frozen gel packs 2,4) none | Days Tested: M-F | Turnaround Time: 1 bus. day | | Comments: | Top of Page | Giemsa Stain | | Fee: 9.50 | Specimen: 1) tissue 2) fluid 3) slide | Shipping Container: 1,2) sterile container 3) slide folder | Coolant: 1,2) frozen gel packs 3) none | Days Tested: M-F | Turnaround Time: 1 bus. day | | Comments: | Top of Page | Gimenez Stain | | Fee: 14.25 | Specimen: 1) tissue 2) fluid 3) slide | Shipping Container: 1,2) sterile container 3) slide folder | Coolant: 1,2) frozen gel packs 3) none | Days Tested: M-F | Turnaround Time: 1 bus. day | | Comments: | Top of Page | Gram Stain | | Fee: 9.50 | Specimen: 1) tissue 2) fluid 3) slide | Shipping Container: 1,2) sterile container 3) slide folder | Coolant: 1,2) frozen gel packs 3) none | Days Tested: M-F | Turnaround Time: 1 bus. day | | Comments: | Top of Page | Isolate Identification | Fee: 1) 34.75 2) 47.50 | Specimen: 1) pure culture 2) impure culture | Shipping Container: 1,2) agar plate or slant | Coolant: none | Days Tested: M-F | Turnaround Time: 5 bus. days | | Comments: Prevent agar from freezing. | Top of Page | Lawsonia intracellularis (PCR), Equine | | Fee: 32.50 | Specimen: 1) ileum 2) feces | Shipping Container: 1,2) sterile container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 7 bus. days | | Comments: | Top of Page | Lawsonia intracellularis (PCR), Porcine | | Fee: 32.50 | Specimen: 1) ileum 2) feces | Shipping Container: 1,2) sterile container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 7 bus. days | | Comments: | Top of Page | Listeria Culture | | Fee: 23.75 | Specimen: 1) tissue 2) silage | Shipping Container: 1) sterile container 2) clean, sealed container | Coolant: 1) frozen gel packs 2) none | Days Tested: M-F | Turnaround Time: 4-30 bus. days | | Comments: | Top of Page | Mycobacterium paratuberculosis - Johne's Culture | | Fee: 27.00 | Specimen: five grams of feces | Shipping Container: tightly covered, clean container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 16 weeks | | Comments: Please call to schedule. | Top of Page | Mycobacterium paratuberculosis - Johne's (PCR) | | Fee: 21.75 | Specimen: feces (no more than 10g needed) | Shipping Container: Do NOT use GLOVE or WHIRL-PAC BAG as container. Use leakproof hard plastic container with screw cap | Coolant: frozen gel packs or dry ice | Days Tested: T,Th,F | Turnaround Time: 3-7 bus. days | | Comments: Please make sure container has no outside contamination. NO GLOVES or WHIRL PAC BAGS. Specimens from sheep, goat and exotic ruminants also accepted. | Top of Page | Mycoplasma hyopneumoniae Culture in Broth | | Fee: 105.00 | Specimen: 1) bronchial swab or lavage 2) lung tissue | Shipping Container: 1) plastic tube 2) sterile container | Coolant: frozen gel packs | Days Tested: as needed | Turnaround Time: 4-5 weeks | | Comments: Please call to schedule. More information. | Top of Page Top of Page | Mycoplasma spp. Culture | | Fee: 30.00 | Specimen: 1) uterine, cervical or vaginal mucus 2) semen 3) lung tissue 4) tracheal wash 5) fluid | Shipping Container: 1,2,4,5) Amies transport media 3) sterile container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 14 bus. days | | Comments: Ship overnight or deliver the same bus. day. | Top of Page | Salmonella spp. Culture | | Fee: 21.50 | Specimen: 1) tissue 2) feces | Shipping Container: 1,2) clean container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 2-5 bus. days | | Comments: | Top of Page
| Salmonella spp. Culture - Primary Culture** | | Fee: 22.00 | Specimen: 1) environmental samples 2) cloacal swab 3) whole bird | Shipping Container: 1) 18 oz. specimen bag 2) lab provided 3) clean container | Coolant: frozen gel packs and cooler | Days Tested: M-W | Turnaround Time: 4 weeks | | Comments: |
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| Salmonella spp. Restart Culture** | | Fee: 11.00 | Specimen: culture | Shipping Container: agar plate or slant | Coolant: none | Days Tested: M-Th | Turnaround Time: 4 weeks | | Comments: |
Top of Page | Salmonella spp. Screen (Delayed Enrichment) | | Fee: 25.75 | Specimen: 1) chicks 2) feed 3) environmental samples | Shipping Container: 1,2,3) clean container (well labeled) | Coolant: 1) frozen gel packs 2,3) none | Days Tested: M-F | Turnaround Time: 5-14 bus. days | | Comments: | Top of Page
| Salmonella spp. Culture (Delayed Enrichment) - Secondary Culture** | | Fee: 25.75 | Specimen: 1) environmental samples 2) cloacal swab 3) whole bird | Shipping Container: 1) 18 oz. specimen bag 2) lab provided 3) clean container | Coolant: frozen gel packs and cooler | Days Tested: M-W | Turnaround Time: 4 weeks | | Comments: |
Top of Page | Salmonella spp. Screen (Eggs APHIS "Egg Sampling Method") | | Fee: 29.00/pool of 20 | Specimen: eggs | Shipping Container: clean container (well labeled) | Coolant: none | Days Tested: M-F | Turnaround Time: 5-7 bus. days | | Comments: Prevent eggs from freezing or breaking. Submission of extra eggs is recommended to account for breakage during shipment. | Top of Page | Salmonella spp. Serogrouping | | Fee: 19.00 | Specimen: pure culture | Shipping Container: agar plate or slant | Coolant: none | Days Tested: M-F | Turnaround Time: 1-2 bus. days | | Comments: Prevent agar from freezing | Top of Page
| Salmonella spp. Serogrouping** | | Fee: 8.00 | Specimen: pure culture | Shipping Container: agar plate or slant | Coolant: none | Days Tested: M-F | Turnaround Time: 4 weeks | | Comments: |
Top of Page | Salmonella spp. Serotyping | Fee: No charge for diagnostic isolates (see comments) | Specimen: pure culture | Shipping Container: agar plate or slant | Coolant: none | Days Tested: M-F | Turnaround Time: 4-6 weeks | | Comments: $38.00 charge for research and environmental isolates. Prevent agar from freezing. | Top of Page
| Salmonella spp. Serotyping** | | Fee: 13.25 | Specimen: pure culture | Shipping Container: agar plate or slant | Coolant: none | Days Tested: M-F | Turnaround Time: 4-6 weeks | | Comments: |
Top of Page | Serotyping (Actinobacillus pleuropneumoniae, Streptococcus suis, Haemophilus parasuis) | | Fee: ref. lab fee | Specimen: pure culture | Shipping Container: agar plate or slant | Coolant: none | Days Tested: M-F | Turnaround Time: 2-3 weeks | | Comments: Prevent agar from freezing. | Top of Page | Susceptibility Testing/MIC | | Fee: 19.00 | Specimen: 1) tissue 2) swab 3) pure culture | Shipping Container: 1) sterile container 2) culturette 3) agar plate or slant | Coolant: 1,2) frozen gel packs 3) none | Days Tested: M-F | Turnaround Time: 2-4 bus. days | | Comments: | Top of Page Top of Page | Ureaplasma spp. Culture | | Fee: 26.25 | Specimen: 1) uterine, cervical or vaginal mucus 2) semen 3) lung tissue 4) tracheal wash 5) fluid | Shipping Container: 1,2,4,5) Amies transport media 3) sterile container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 7 bus. days | | Comments: Ship overnight or deliver the same day. | Top of Page | Urolith Culture | | Fee: 71.50 | Specimen: urolith | Shipping Container: clean container | Coolant: none | Days Tested: M-F | Turnaround Time: 3-4 bus. days | | Comments: | Top of Page | Water Culture (includes counts on total Gram negative, Gram negative lactose fermenters (coliforms), Gram negative non-lactose fermenters, and Pseudomonas aeruginosa.) | | Fee: 38.75 | Specimen: 200 ml water | Shipping Container: sterile container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 4 bus. days | | Comments: Keep chilled. | Top of Page | Yersinia Culture | | Fee: 26.25 | Specimen: 1) feces 2) tissue | Shipping Container: 1,2) sterile container | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: 2-4 bus. days | | Comments: | Top of Page Note on days tested and turnaround times: Samples must be received at least 1 business day before the scheduled testing day in order to be tested on that day. Samples may not always be run on the first scheduled testing day after they arrive, but testing will be completed within the posted turnaound time. If rush testing is desired, please contact us. Top of Page
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