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  Home > Services and Fees > Bacterial PCR
 

Bacterial PCR

Effective February 13, 2014 


Note: A $10 accession fee will be applied to all submissions. All fees are in US $.

 

Note on days tested and turnaround times:  Samples must be received at least 1 business day before the scheduled testing day.  New client accounts can take up to 2-3 business days to establish before testing can begin.  Samples may not always be tested on the first scheduled day after they arrive; however, it is our goal to complete testing within the posted turnaround time.  If rush testing is desired, please contact us.

Pooling any sample may produce a false negative result.  Pooling strategies should consider the characteristics/pathogenesis of the disease and test being used.

 

Click the name of the test or procedure for detailed information.

 

 

Actinobacillus pleuropneumoniae - APP
Fee:  37.50

Specimen:

1) tonsillar swab
2) whole tonsil
3) lung

Shipping Container:

1) swab
2,3) tissue

Coolant:

frozen gel packs

Days Tested:

Turnaround Time:

2-10 bus. days 

Comments: Results reported on Tuesdays. Additional information.

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Actinobacillus pleuropneumoniae - APP (Serotypes 3, 6 and 8)
Fee:  37.50

Specimen:

1) tonsillar swab
2) whole tonsil
3) lung

Shipping Container:

1) swab
2,3) tissue

Coolant:

frozen gel packs

Days Tested:

M-F 

Turnaround Time:

2-10 bus. days

Comments: Testing upon request.

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Actinobacillus pleuropneumoniae/Actinobacillus suis Multiplex - APP/A. suis
Fee:  37.50

Specimen:

1) tonsillar swab
2) whole tonsil
3) lung

Shipping Container:

1) swab
2,3) tissue

Coolant:

frozen gel packs

Days Tested:

M

Turnaround Time:

2-10 bus. days

Comments: Results reported on Tuesdays.

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Actinobacillus pleuropneumoniae - APP Serotyping
Fee:  37.50

Specimen:

bacterial isolate

Shipping Container:

culture media

Coolant:

none

Days Tested:

T

Turnaround Time:

2-10 bus. days

Comments: Results reported on Wednesdays. Rush testing available upon request.

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Actinobacillus suis
Fee:  37.50

Specimen:

1) tonsillar swab
2) whole tonsil
3) lung

Shipping Container:

1) swab
2,3) tissue

Coolant:

frozen gel packs

Days Tested:

M

Turnaround Time:

2-10 bus. days

Comments: Results reported on Tuesdays. Additional information.

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Bacillus anthracis
Fee:  no charge

Specimen:

1) bacterial cultures
2) blood
3) tissue

Shipping Container:

1) leakproof container
2) tube
3) whirl pak or plastic bag

Coolant:

frozen gel packs

Days Tested:

as requested

Turnaround Time:

7 bus. days

Comments: Testing is completed at the MN Department of Health

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Bacterial Identification by DNA Sequencing (16S rRNA gene)
Fee:  50.00

Specimen:

bacterial isolate

Shipping Container:

culture media

Coolant:

none

Days Tested:

T

Turnaround Time:

1-2 weeks

Comments: Results reported on Thursdays.  Additional information.

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Brachyspira hyodysenteriae and Brachyspira pilosicoli Duplex PCR
Fee:  37.50

Specimen:

 

1) Bacterial isolate
2) Feces
3) Fecal swabs
4) Colon

 

 

Shipping Container:        

 1) Culture media in leak proof container
2) Leak proof container
3) Swab
4) Whirl Pak or secure plastic bag

 

Coolant:

none

Days Tested:

W

Turnaround Time:

2-10 bus. days

Comments: Results reported on Thursdays.

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Brachyspira Sequencing PCR
Fee:  37.50

Specimen:

Brachyspira isolate

Shipping Container:

culture media

Coolant:

none

Days Tested:

T

Turnaround Time:

1-2 weeks

Comments: Results reported on Thursdays.

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Clostridium perfringens Toxin Gene Typing
Fee:  34.00

Specimen:

bacterial isolate

Shipping Container:

culture plate

Coolant:

none

Days Tested:

W

Turnaround Time:

2-10 bus. days

Comments: Results reported on Thursdays.

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Erysipelothrix rhusiopathiae Real Time PCR
Fee:  37.50

Specimen:

1) skin lesions
2) systemic tissues
3) fetuses

Shipping Container:

tissue

Coolant:

frozen gel packs

Days Tested:

M, Th

Turnaround Time:

2-10 bus. days

Comments: Results reported on Tuesdays and Fridays. Additional information.

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Escherichia coli Virulence Factors
Fee:  33.25

Specimen:

bacterial isolate

Shipping Container:

culture media

Coolant:

none

Days Tested:

M,Th

Turnaround Time:

2-10 bus. days

Comments: Results reported on Tuesdays and Fridays.

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Fish Barcode DNA Sequencing for Species Identification
Fee:  50.00

Specimen:

muscle tissue

 

Shipping Container:

1.5 mL tube containing alcohol
 

 

Coolant:

none

 

Days Tested:

T

Turnaround Time:

1-2 weeks

Comments:  Results are reported on Fridays.

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Genotype Analysis, Bacterial (BioNumerics)
Fee:  37.50

Specimen:

bacterial isolate

Shipping Container:

culture media

Coolant:

none

Days Tested:

W

Turnaround Time:

2-4 weeks

Comments: Results reported on Tuesdays.

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Haemophilus parasuis Real Time PCR
Fee:  37.50

Specimen:

systemic tissues (fibrin)

Shipping Container:

1) swabs
2) tissues
3) fluids

Coolant:

frozen gel packs

Days Tested:

M,Th

Turnaround Time:

2-10 bus. days

Comments: Results reported on Tuesdays and Fridays. Additional information.

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Lawsonia intracellularis Real Time PCR
Fee:  32.50

Specimen:

1) ileum
2) feces
3) swabs (fecal or rectal)

Shipping Container:

1) sterile container
2) Do NOT use GLOVE or WHIRL-PAC BAG as container. Use leakproof hard plastic container with screw cap.
3) synthetic swab

Coolant:

frozen gel packs

Days Tested:

M,Th

Turnaround Time:

7 bus. days

Comments: Results reported on Tuesdays and Fridays.
2) Please make sure container has no outside contamination. NO GLOVES or WHIRL-PAC BAGS. 

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Leptospira sp.
Fee:  18.75

Specimen:

1) 10 ml urine
2) tissue (fetuses, kidney)
3) 100 ml water

Shipping Container:

1) vial
2) plastic bag
3) leakproof container

Coolant:

frozen gel packs

Days Tested:

W

Turnaround Time:

2-10 bus. days

Comments: Results reported on Fridays.

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Mycobacterium paratuberculosis - Johne's
Fee:  21.75

Specimen:

feces (no more than 10 g needed)

Shipping Container:

Do NOT use GLOVE or WHIRL-PAC BAG as container. Use leakproof hard plastic container with screw cap.

Coolant:

frozen gel packs or dry ice

Days Tested:

F

Turnaround Time:

3 bus. days-1 week

Comments: Please make sure container has no outside contamination. NO GLOVES or WHIRL-PAC BAGS. Specimens from sheep, goat and exotic ruminants also accepted.

Ct Values from this assay correspond to the shedding status of the animal as follows:
Less than Ct 25 = High Shedding
Ct 25 to Ct 30 = Moderate Shedding
Ct 30 to Ct 37 = Low Shedding
Ct 37 to Ct 40 = Suspect Animal
Above 40 or no Ct value = Negative

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Mycoplasma bovis Real Time PCR
Fee:  23.75

Specimen:

1) tissue homogenates
2) lung tissue
3) bacterial isolates
4) joint swab
5) other

Shipping Container:

1,2) vial or leakproof container
3) culture media
4) synthetic swab

 

Coolant:

Frozen gel packs

 

 

Days Tested:

Th

Turnaround Time:

2-8 bus. days

Comments:  Results reported on Thursdays.

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Mycoplasma gallisepticum (PCR)

Fee:  27.50

Specimen:

1) swab in sp4 media
2) swab (tracheal, joint or airsac)

Shipping Container:

1) vial or leakproof container
2) synthetic swab or plastic bag

Coolant:

frozen gel packs

Days Tested:

T,Th

Turnaround Time:

2-6 bus. days

Comments: Results reported on Wednesdays and Fridays. Samples must be received at least the day before test date. Please contact the lab if you need rush testing. If culture is desired, sp4 media should be used for specimen shipment. Click Here to order media.  Additional Information.

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Mycoplasma hyopneumoniae (Applied Biosystems)
Fee:  30.25

Specimen:

1) swab (nasal, tracheal or bronchial)
2) lung
3) oral fluids/saliva

Shipping Container:

1) culturette
2) whirl-pac or plastic bag
3) sterile tube

Coolant:

frozen gel packs

Days Tested:

M-F

Turnaround Time:

2-10 bus. days

Comments:

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Mycoplasma hyopneumoniae Complete P146 Sequencing
Fee:  149.00

Specimen:

1) swabs (nasal, tracheal, or bronchial)*
2) lung*
3) isolate
4) oral fluids/saliva

*Positive for M. hyo. by PCR

Shipping Container:

1) synthetic swab
2) whirl-pac or plastic bag
3,4) sterile tube
 

Coolant:

frozen gel packs

Days Tested:

weekly

Turnaround Time:

2 weeks

Comments:

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Mycoplasma hyorhinis Real Time PCR
Fee:  18.75

Specimen:

1) joint swab
2) joint fluid
3) synovial membrane

Shipping Container:

1) swab
2) tissue

Coolant:

frozen gel packs

Days Tested:

M, Th

Turnaround Time:

2-10 bus. days

Comments: Results reported on Tuesdays and Fridays.

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Mycoplasma hyosynoviae Real Time PCR
Fee:  18.75

Specimen:

1) joint swab
2) joint fluid
3) synovial membrane

Shipping Container:

1) swab
2) tissue

Coolant:

frozen gel packs

Days Tested:

M, Th

Turnaround Time:

2-10 bus. days

Comments: Results reported on Tuesdays and Fridays.

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Mycoplasma iowae (PCR)
Fee:  27.50

Specimen:

1) swab in sp4 media
2) swab (tracheal, joint or airsac)

Shipping Container:

1) vial or leakproof container
2) synthetic swab or plastic bag

Coolant:

frozen gel packs

Days Tested:

T,Th

Turnaround Time:

2-6 bus. days

Comments: Results reported on Wednesdays and Fridays. Samples must be received at least the day before test date. Please contact the lab if you need rush testing. If culture is desired, sp4 media should be used for specimen shipment. Click Here to order media.  Additional Information.

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Mycoplasma meleagridis (PCR)
Fee:  27.50

Specimen:

1) swab in sp4 media
2) swab (tracheal, joint or airsac)

Shipping Container:

1) vial or leakproof container
2) synthetic swab or plastic bag

Coolant:

frozen gel packs

Days Tested:

T,Th

Turnaround Time:

2-6 bus. days

Comments: Results reported on Wednesdays and Fridays. Samples must be received at least the day before test date. Please contact the lab if you need rush testing. If culture is desired, sp4 media should be used for specimen shipment. Click Here to order media.  Additional Information.

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Mycoplasma Speciation
Fee:  50.00

Specimen:

bacterial isolate

Shipping Container:

culture media

Coolant:

none

Days Tested:

W

Turnaround Time:

1-2 weeks

Comments: Results reported on Thursdays. Additional information.

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Mycoplasma synoviae (PCR)
Fee:  27.50

Specimen:

1) swab in sp4 media
2) swab (tracheal, joint or airsac)

Shipping Container:

1) vial or leakproof container
2) synthetic swab or plastic bag

Coolant:

frozen gel packs

Days Tested:

T,Th

Turnaround Time:

2-6 bus. days

Comments: Results reported on Wednesdays and Fridays. Samples must be received at least the day before test date. Please contact the lab if you need rush testing. If culture is desired, sp4 media should be used for specimen shipment. Click Here to order media.  Additional Information.

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Pasteurella multocida Toxin Testing
Fee:  37.50

Specimen:

1) nasal swabs
2) P. multocida isolate

Shipping Container:

1) swab
2) culture media

Coolant:

frozen gel pack

Days Tested:

M

Turnaround Time:

2-10 bus. days

Comments: Results reported on Tuesdays. Rush testing available.

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Salmonella cholerasuis Argus-Vaccine Identification
Fee:  32.50

Specimen:

bacterial isolate

Shipping Container:

culture media

Coolant:

none

Days Tested:

W

Turnaround Time:

2-10 bus. days

Comments: Results reported on Thursdays.

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Salmonella enterica Real-time PCR (TaqMan®)
Fee:  31.25

Specimen:

1) tissue
2) feces (no more than 10g needed)

Shipping Container:

Do NOT use GLOVE or WHIRL-PAC BAG as container. Use leakproof hard plastic container with screw cap.

Coolant:

frozen gel packs or dry ice

Days Tested:

Th

Turnaround Time:

3-7 bus. days

Comments: Please make sure container has no outside contamination. NO GLOVES or WHIRL-PAC BAGS.

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Streptococcus suis
Fee:  35.50

Specimen:

systemic tissues
 

Shipping Container:

1) swabs
2) tissues
3) fluids

Coolant:

frozen gel packs

Days Tested:

M

Turnaround Time:

2-10 bus. days

Comments: Results are reported on Tuesdays.

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Streptococcus suis Serotyping (Serotypes 1, 2, 7 and 9)
Fee:  37.50

Specimen:

bacterial isolate
 

Shipping Container:

culture media

Coolant:

none

Days Tested:

W

Turnaround Time:

2-10 bus. days

Comments: Results are reported on Thursdays.

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