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  Home > Services and Fees > Viral PCR
 

Viral PCR

Effective March 15, 2014 

Note: A $10 accession fee  will be applied to all submissions. All fees are in US $.

 

Note on days tested and turnaround times:  Samples must be received at least 1 business day before the scheduled testing day.  New client accounts can take up to 2-3 business days to establish before testing can begin.  Samples may not always be tested on the first scheduled day after they arrive; however, it is our goal to complete testing within the posted turnaround time.  If rush testing is desired, please contact us.

Pooling any sample may produce a false negative result.  Pooling strategies should consider the characteristics/pathogenesis of the disease and test being used.

 

Click the name of the test or procedure for detailed information.

 

 

Avian Pneumovirus - APV
Fee:  25.00

Specimen:

tracheal swabs

Shipping Container:

15 ml screw cap vial (5 swabs/vial)

Coolant:

frozen gel packs

Days Tested:

W
Turnaround Time:

7 bus. days
Comments: 10 swabs used per test.

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Bovine Coronavirus - BCOV
Fee:  32.50

Specimen:

1) lung, intestine or other tissue homogenate
2) feces
3) fecal or rectal swabs
4) nasal swabs

Shipping Container:

1) leakproof 1.5 ml microfuge tube
2) whirl pac or plastic bag
3,4) synthetic swabs

Coolant:

frozen gel packs

Days Tested:

M,Th
Turnaround Time:

5 bus. days
Comments:

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Bovine Respiratory Syncytial Virus - BRSV
Fee:  23.75

Specimen:

1) lung
2) virus isolate
3) nasal or tracheal swabs

Shipping Container:

1) whirl pac or plastic bag
2) leakproof 1.5 ml microfuge tube
3) synthetic swabs

Coolant:

frozen gel packs, if not frozen previously. If already frozen, send on dry ice

Days Tested:

M,Th
Turnaround Time:

5 bus. days
Comments:

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Bovine Rotavirus (Types A and B)
Fee:  27.00

Specimen:

1) feces
2) intestine
3) feedback material
4) environmental swabs
5) virus isolate

Shipping Container:

1,2,3) whirl-pac or leakpoof container
4) swab
5) 1.5 ml microfuge tube

Coolant:

frozen gel packs

Days Tested:

M,Th
Turnaround Time:

7 bus. days
Comments:

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Bovine Rotavirus A Sequencing (VP7 and VP4)

Fee:  149.00

Specimen: 

 

1) PCR positive sample
2) Virus isolate

   

 

Shipping Container:

2) 1.5 ml microfuge tube   

 

 

Coolant:

Frozen gel packs   

 

 

Days Tested:

M,W,F

Turnaround Time:

2-4 weeks

Comments:

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Bovine Viral Diarrhea - BVD
Fee:  
1) 6.75
2) 17.50

Specimen:

1) serum (at least 0.5 ml)
2) tissue

Shipping Container:

1) leakproof 1.5 ml microfuge tube
2) whirl pac or plastic bag

Coolant:

frozen gel packs

Days Tested:

T,Th
Turnaround Time:

next bus. day (from test date)
Comments:

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Bovine Viral Diarrhea - BVD (for Alpacas and Llamas only)
Fee:  
6.75 (see comments)

Specimen:

1) serum
2) tissue
3) buffy coat

Shipping Container:

1) leakproof 1.5 ml microfuge tube
2) whirl pac or plastic bag
3) 3-5 ml purple top whole blood tube

Coolant:

frozen gel packs

Days Tested:

T,Th
Turnaround Time:

next bus. day
(see comments)
Comments:
Cost is per sample.
Samples will be pooled in groups up to 5 and results will be reported as individual animals.
3) Processing of whole blood samples requires extra time, and could add 1-2 days to the turnaround time of the assay.

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Bovine Viral Diarrhea - BVD Ear Notch Screen Pooled Testing
Fee:  31.25

Specimen:

1cm x 1cm ear tissue
(1/2 inch notching pliers)

Shipping Container:

6 or 10 ml red top tube (no additive or gel)

Coolant:

frozen gel packs

Days Tested:

T,Th
Turnaround Time:

1-2 bus. days
(see comments)
Comments:
Please follow these Submission Guidelines
Samples will be run in pools up to 50.
See Virology for BVD ear notch individual testing.
Processing of ear notch samples requires extra time, and could add 1-2 days to the turnaround time of the assay.

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Bovine Viral Diarrhea - BVD Genetic Analysis
Fee:  149.00

Specimen:

1) serum (at least 300 uL)
2) virus isolate (at least 300 uL)
3) tissue (1 cm3 or biopsy)

Shipping Container:

1,2) 1.5 ml microfuge tube
3) whirl-pac or plastic bag

Coolant:

frozen gel packs

Days Tested:

weekly
Turnaround Time:

2-4 weeks
Comments:
2) Must come with a copy of a USDA shipping permit.
3) Shipping instruction: send next day delivery.

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Circovirus Universal (Types 1 and 2)
Fee:  44.50

Specimen:

1) serum (at least 0.5 ml)
2) tissue
3) feces

Shipping Container:

1) leakproof 1.5 ml microfuge tube
2,3) whirl-pac or plastic bag

Coolant:

frozen gel packs

Days Tested:

T,F
Turnaround Time:

1-2 bus. days (from test date)
Comments:

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Circovirus Type 2
Fee:  37.25

Specimen:

1) semen (at least 0.5 ml)
2) serum (at least 0.5 ml)
3) tissue
4) blood swabs
5) feces
6) oral fluids/saliva (at least 2 ml)

Shipping Container:

1,4) leakproof 1.5 ml microfuge tube
2) red top tube
3,5) whirl-pac or plastic bag
6) sterile tube or whirl pac
 

Coolant:

frozen gel packs

Days Tested:

T,F
Turnaround Time:

1-2 bus. days (from test date)
Comments: 6) Do not pool group/pen samples.

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Circovirus Type 2 Genetic Analysis
Fee:  121.75

Specimen:

1) serum (at least 300 uL)
2) virus isolate (at least 300 uL)
3) tissue (1 cm3 or biopsy)
4) oral fluids/saliva (at least 2 ml)

Shipping Container:

1,2) 1.5 ml microfuge tube
3) whirl-pac or plastic bag
4) sterile tube or whirl pac

Coolant:

frozen gel packs

Days Tested:

weekly
Turnaround Time:

2-4 weeks
Comments:
2) Must come with a copy of a USDA shipping permit.
3) Shipping instruction: send next day delivery.
4) Do not pool group/pen samples.

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Encephalomyocarditis Virus - EMC
Fee:  32.50

Specimen:

1) tissue
2) virus isolate

Shipping Container:

1) whirl-pac or plastic bag
2) leakproof 1.5 ml microfuge tube

Coolant:

frozen gel packs

Days Tested:

biweekly (Th)
Turnaround Time:

no more than 14 bus. days
Comments:

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Equine Encephalidites
Fee:  no charge

Specimen:

brain tissue

Shipping Container:

 

Coolant:

 

Days Tested:

daily
Turnaround Time:

4 bus. days
Comments: Sent to Minnesota Dept. of Health.

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Exotic Newcastle Disease Matrix (USDA)
Fee:  39.50

Specimen:

tracheal swabs

Shipping Container:

synthetic swabs

Coolant:

frozen gel packs

Days Tested:

W
Turnaround Time:

1-2 bus. days (from test date)
Comments: Swabs may be pooled up to 5.

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Exotic Newcastle Disease Fusion (USDA)
Fee:  39.50

Specimen:

tracheal swabs

Shipping Container:

synthetic swabs

Coolant:

frozen gel packs

Days Tested:

W
Turnaround Time:

1-2 bus. days (from test date)
Comments: Swabs may be pooled up to 5. Fusion PCR is run to detect virulent NDV after a positive is obtained by END virus Matrix PCR testing.

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Hemagglutinating Encephalomyelitis Virus - HEV (VWD)
Fee:  32.50

Specimen:

 

1) tissue
2) virus isolate
3) oral fluids

 

Shipping Container:

1) whirl-pac or plastic bag
2,3) leakproof 1.5 ml microfuge tube

Coolant:

frozen gel packs

Days Tested:

M,Th

Turnaround Time:

5 bus. days
Comments:

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Hemagglutinating Encephalomyelitis Virus (VWD) Full Length HE Gene Sequencing
Fee:  149.00

Specimen:

 

1) tissue
2) virus isolate
3) oral fluids

 

Shipping Container:

 

1) whirl-pac or plastic bag
2,3) leakproof 1.5 ml microfuge tube

 

 

Coolant:

frozen gel packs

 

 

Days Tested:

as needed

Turnaround Time:

2-4 weeks

Comments:

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Infectious Bovine Rhinotracheitis - IBR/BHV 1
Fee:  23.75

Specimen:

1) swabs (nasal, conjunctival, vaginal or preputial)
2) lung or other tissue
3) virus isolate
4) tracheal wash or lavage

Shipping Container:

1) synthetic swabs
2) whirl-pac or plastic bag
3) leakproof 1.5 ml microfuge tube
4) sterile tube
 

Coolant:

1,2,4) frozen gel packs
3) dry ice

Days Tested:

T
Turnaround Time:

same day (as test date) 
Comments: Results reported on Tuesdays (samples must be recieved at least the day before test date).

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Influenza Virus for Routine Testing - Group A Matrix RRT - PCR
Fee:  30.00

Specimen:

1) nasal or pharyngeal swabs
2) lung (2-3 cm3 or biopsy)
3) oral fluids/saliva (at least 2 ml)

Shipping Container:

1) synthetic swabs
2,3) whirl-pac or plastic bag

Coolant:

frozen gel packs

Days Tested:

M-F
Turnaround Time:

2-3 bus. days

Comments:
1) For mammals
2) Shipping instruction: send next day delivery.
3) Do not pool group/pen samples.

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Influenza Virus - Group A Matrix (USDA)
Fee:  30.00

Specimen:

swabs

Shipping Container:

synthetic swabs

Coolant:

frozen gel packs

Days Tested:

M-F
Turnaround Time:

2 bus. days
Comments: For Avians. Swabs may be pooled up to 5. Results reported same day as test date.

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Influenza Virus - Group A, Subtyping for H & N
Fee:  22.00

Specimen:

1) nasal or pharyngeal swabs
2) virus isolate (at least 0.5 ml)
3) lung (2-3 cm3 or biopsy)
4) oral fluids/saliva (at least 2 ml)
 

Shipping Container:

1) synthetic swabs
2) leakproof 1.5 ml microfuge tube
3) whirl-pac or plastic bag

Coolant:

1,3) frozen gel packs
2) dry ice

Days Tested:

T,Th
Turnaround Time:

1-2 weeks
Comments:
Subtyping tests are run on samples previously testing positive for influenza virus.
2) Must come with a copy of an USDA shipping permit.
3) Shipping instruction: send next day delivery.
4) Do not pool group/pen samples.

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Influenza Virus - Group A, Subtyping for H5/H7 (USDA)
Fee:  15.25

Specimen:

swabs

Shipping Container:

synthetic swabs

Coolant:

frozen gel packs

Days Tested:

M,W
Turnaround Time:

24 hrs. (from test date)
Comments: Testing for subtyping is run after a positive is obtained by Influenza virus Matrix testing.

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Influenza Virus - USDA Differential N1
Fee:  no charge

Specimen:

1) matrix influenza PCR positive sample
2) virus isolate
 

Shipping Container:

1) sterile tube
2) 1.5 ml microfuge tube

Coolant:

frozen gel packs

Days Tested:

Turnaround Time:

Comments: Requires a written agreement with the USDA.

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Influenza Virus Sequencing
Fee:  149.00

Specimen:

1) nasal swabs
2) viral isolate (at least 0.5 ml)
3) lung (2-3 cm3 or biopsy)
4) oral fluids/saliva (at least 2 ml)

Shipping Container:

1) synthetic swabs
2) leakproof 1.5 ml microfuge tube
3,4) whirl-pac or plastic bag

Coolant:

1,3,4) frozen gel packs
2) dry ice

Days Tested:

weekly
Turnaround Time:

2-4 weeks
Comments:
2) Must come with a copy of an USDA shipping permit.
3) Shipping instruction: send next day delivery.
4) Do not pool group/pen samples.

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Koi Herpes Virus
Fee:  26.25

Specimen:

1) tissue
2) culture media
 

Shipping Container:

sterile container

Coolant:

frozen gel packs

Days Tested:

as needed
Turnaround Time:

3-5 bus. days
Comments:

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Porcine Cytomegalovirus - PCMV
Fee:  62.00

Specimen:

1) nasal turbinates
2) nasal swabs
3) buffy coat

Shipping Container:

 

Coolant:

frozen gel packs

Days Tested:

T
Turnaround Time:

1-2 weeks
Comments:

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Porcine Epidemic Diarrhea Virus ( PEDV) and Transmissible Gastroenteritis Virus (TGEV) Real Time Multiplex PCR
Fee:  30.00

Specimen:

1) feces
2) fecal swab
3) fresh small intestine
4) oral fluids
5) feed 

Shipping Container:

1,3) whirl-pac or leakproof container
2) swab 
4) sterile tube 

Coolant:

Frozen gel packs

 

Days Tested:

M-F

Turnaround Time:

24 working hours

Comments: 5) 5) feed sample results reporting 3-4 working days.

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Porcine Epidemic Diarrhea (PEDV) Whole Genome Next Gen Sequencing
Fee:  300.00

Specimen:

1) feces
2) fecal swab
3) fresh small intestine
4) viral isolate

 

Shipping Container:

1,3) whirl-pac or leakproof container
2) swab

 

 

Coolant:

frozen gel packs

 

 

Days Tested:

as needed

Turnaround Time:

2-4 weeks

Comments:  For optimal result, sequencing should only be requested on highly positive samples to Ct values of 25 or less.

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Porcine Parvovirus and Parvovirus 2 - (PPV) Real Time PCR
Fee:  32.50

Specimen:

1) semen (at least 0.5 ml)
2) serum (at least 0.5 ml)
3) buffy coat
4) tissue
5) blood swabs
6) feces
7) oral fluids

 

 

Shipping Container:

1,2,5) leakproof 1.5 ml microfuge tube or serum separator tube
3) 3-5 ml purple top whole blood tube
4,6) whirl-pac or plastic bag
7) sterile tube

Coolant:

frozen gel packs

Days Tested:

W
 

Turnaround Time:

1-2 bus. days (from test date)

 

Comments: A maximum of 5 samples/test will be pooled for serum, semen, or blood swab testing.

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Porcine Reproductive and Respiratory Virus - NAEU PRRS, Life Tech
Fee:  28.00

Specimen:

1) semen (at least 1 ml)
2) serum (at least 0.5 ml)
3) tissue (1 cm3 or biopsy)
4) blood swabs
5) nasal swabs
6) oral fluids/saliva (at least 2 ml)

Shipping Container:

1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl-pac or plastic bag
5) synthetic swabs
6) sterile tube or whirl pac

Coolant:

frozen gel packs

Days Tested:

M-F

Turnaround Time:

same day (boar stud monitoring)
or next bus. day (regular request)

Holiday schedule for boar stud monitoring

Comments:
3) Shipping instruction: send next day delivery.
6) Do not pool group/pen samples.
The Veterinary Diagnostic Laboratory prefers pooling samples at the lab. Additional information.

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Porcine Reproductive and Respiratory Virus - PRRS, Quantitative (U.S. Strain Only)
Fee:  32.50

Specimen:

1) semen (at least 1 ml)
2) serum (at least 0.5 ml)
3) tissue (1 cm3 or biopsy)

Shipping Container:

1,2) 1.5 ml microfuge tube
3) whirl-pac or plastic bag

Coolant:

frozen gel packs

Days Tested:

T
Turnaround Time:

1-2 bus. days (from test date)
Comments:
3) Shipping instruction: send next day delivery.
Additional information.

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Porcine Reproductive and Respiratory Virus - PRRS, Genetic Analysis (Strain Differentiating/Sequencing)
Fee:  135.25

Specimen:

1) serum (at least 300 uL)
2) virus isolate (at least 300 uL)
3) tissue (1 cm3 or biopsy)
4) extracted RNA
5) oral fluids/saliva (at least 2 ml)

Shipping Container:

1,2) 1.5 ml microfuge tube
3) whirl-pac or plastic bag
4) sterile tube
5) sterile tube or whirl pac

Coolant:

frozen gel packs

Days Tested:

weekly
Turnaround Time:

2-4 weeks
Comments:
2) Must come with a copy of a USDA shipping permit.
3) Shipping instruction: send next day delivery.
5) Do not pool group/pen samples.
Additional info.

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Porcine Reproductive and Respiratory Virus - PRRS, Tetracore EZ-PRRSV MPX 4.0 Real Time Multiplex
Fee:  48.75

Specimen:

1) semen (at least 1 ml)
2) serum (at least 0.5 ml)
3) tissue
4) blood swabs

Shipping Container:

1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl-pac or plastic bag

Coolant:

frozen gel packs

Days Tested:

as needed
Turnaround Time:

1 bus. day (from test day)
Comments: 3) Shipping instruction: send next day delivery. Additional Information.

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Porcine Rotavirus (Types A, B and C)
Fee:  27.00

Specimen:

1) feces
2) intestine
3) feedback material
4) environmental swabs
5) virus isolate

Shipping Container:

1,2,3) whirl-pac or leakpoof container
4) swab
5) 1.5 ml microfuge tube

Coolant:

frozen gel packs

Days Tested:

T, Th
Turnaround Time:

3 bus. days
Comments:

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Porcine Rotavirus A Sequencing (VP7 and VP4)
Fee:  149.00

Specimen:

1) PCR positive sample
2) virus isolate

Shipping Container:

2) 1.5 ml microfuge tube

Coolant:

frozen gel packs

Days Tested:

M,W,F
Turnaround Time:

2-4 weeks
Comments:

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Porcine Rotavirus B Sequencing (VP7)
Fee:  135.25

Specimen:

 

1) PCR positive sample
2) Virus isolate

 

 

Shipping Container:

2) 1.5 ml microfuge tube

 

 

Coolant:

Frozen gel packs

 

 

 Days Tested:

M,W,F

Turnaround Time:

2-4 weeks

  Comments:

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Porcine Rotavirus C Sequencing (VP7)
Fee:  135.25

Specimen:

 

1) PCR positive sample
2) Virus isolate

 

 

Shipping Container:

2) 1.5 ml microfuge tube

 

 

Coolant:

Frozen gel packs

 

 

 Days Tested:

M,W,F

Turnaround Time:

2-4 weeks

Comments:

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Spring Viremia of Carp
Fee:  29.00

Specimen:

1) tissue
2) culture media
 

Shipping Container:

sterile container

Coolant:

frozen gel packs

Days Tested:

as needed
Turnaround Time:

3-5 bus. days
Comments:

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Swine Hepatitis E Virus
Fee:  59.25

Specimen:

1) Feces
2) Fecal swabs
3) Fresh small intestine
4) Fresh liver tissue


 

Shipping Container:

1) Whirl pac or plastic bag
2) Synthetic swabs
3) Leak proof container
4) Leak proof container

 

Coolant:

 

Frozen gel packs,
dry ice if already frozen

 

 

 

Days Tested:

T

Turnaround Time:

1-2 weeks

Comments:

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Swine Delta Coronavirus Real time PCR - SDCV
Fee:  28.00

Specimen:
1) intestine or other tissue homogenate
2) feces
3) fecal swabs
4) oral fluids
5) enviromental samples
6) feed

Shipping Container:

1,2,6) whirl pac or plastic bag
2) leakproof 1.5 ml microfuge tube
3) synthetic swabs
4) leakproof 4 mL, 15 mL or 50 mL tube
5) swiffers or synthetic swabs

Coolant:

frozen gel packs

 

Days Tested:

M, W, F

Turnaround Time:

5 bus. days

Comments:

 

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Transmissible Gastroenteritis Virus (TGEV) Full Length Spike Gene Sequencing
Fee:  447.00

Specimen:

 

1) feces
2) fecal swab
3) fresh small intestine
4) viral isolate

 

 

 

Shipping Container:

 

1,3) whirl-pac or leakproof container
2) swab

 

   

 

 

Coolant:

frozen gel packs

 

 

 

 

Days Tested:

as needed

Turnaround Time:

2-4 weeks

Comments:

Top of Page

Transmissible Gastroenteritis Virus (TGEV) ORF3 Sequencing
Fee:  135.25

Specimen:

 

1) feces
2) fecal swab
3) fresh small intestine
4) viral isolate

 

Shipping Container:

 

1,3) whirl-pac or leakproof container
2) swab

 

 

 

Coolant:

frozen gel packs

 

 

 

Days Tested:

as needed

Turnaround Time:

2-4 weeks

Comments:

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Viral Hemorrhagic Septicemia
Fee:  10.50

Specimen:

1) tissue
2) culture media
 

Shipping Container:

sterile container

Coolant:

frozen gel packs

Days Tested:

F
Turnaround Time:

7 bus. days
Comments: RT-PCR or qRT-PCR.  VHS Surveillance Study

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