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Viral PCR
Effective March 8, 2013 | | | Note: A $10 accession fee will be applied to all submissions. All fees are in US $. | | Click the name of the test or procedure for detailed information. | | Avian Pneumovirus - APV | | Fee: 6.75 | Specimen: tracheal swabs | Shipping Container: 15 ml screw cap vial (5 swabs/vial) | Coolant: frozen gel packs | Days Tested: W | Turnaround Time: 7 bus. days | | Comments: 10 swabs used per test. | Top of Page | Bovine Coronavirus - BCOV | | Fee: 32.50 | Specimen: 1) lung, intestine or other tissue homogenate 2) feces 3) fecal or rectal swabs 4) nasal swabs | Shipping Container: 1) leakproof 1.5 ml microfuge tube 2) whirl pac or plastic bag 3,4) synthetic swabs | Coolant: frozen gel packs | Days Tested: M,Th | Turnaround Time: 5 bus. days | | Comments: | Top of Page | Bovine Respiratory Syncytial Virus - BRSV | | Fee: 23.75 | Specimen: 1) lung 2) virus isolate 3) nasal or tracheal swabs | Shipping Container: 1) whirl pac or plastic bag 2) leakproof 1.5 ml microfuge tube 3) synthetic swabs | Coolant: frozen gel packs, if not frozen previously. If already frozen, send on dry ice | Days Tested: M,Th | Turnaround Time: 5 bus. days | | Comments: | Top of Page | Bovine Rotavirus (Types A and B) | | Fee: 27.00 | Specimen: 1) feces 2) intestine 3) feedback material 4) environmental swabs 5) virus isolate | Shipping Container: 1,2,3) whirl-pac or leakpoof container 4) swab 5) 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested: M,Th | Turnaround Time: 7 bus. days | | Comments: | Top of Page | Bovine Rotavirus A Sequencing (VP7 and VP4) | Fee: 149.00 | Specimen: 1) PCR positive sample 2) Virus isolate | Shipping Container: 2) 1.5 ml microfuge tube | Coolant: Frozen gel packs | Days Tested: M,W,F | Turnaround Time: 2-4 weeks | | Comments: | Top of Page | Bovine Viral Diarrhea - BVD | Fee: 1) 6.75 2) 17.50 | Specimen: 1) serum (at least 0.5 ml) 2) tissue | Shipping Container: 1) leakproof 1.5 ml microfuge tube 2) whirl pac or plastic bag | Coolant: frozen gel packs | Days Tested: T,Th | Turnaround Time: next bus. day (from test date) | | Comments: | Top of Page | Bovine Viral Diarrhea - BVD (for Alpacas and Llamas only) | Fee: 6.75 (see comments) | Specimen: 1) serum 2) tissue 3) buffy coat | Shipping Container: 1) leakproof 1.5 ml microfuge tube 2) whirl pac or plastic bag 3) 3-5 ml purple top whole blood tube | Coolant: frozen gel packs | Days Tested: T,Th | Turnaround Time: next bus. day (see comments) | Comments: Cost is per sample. Samples will be pooled in groups up to 5 and results will be reported as individual animals. 3) Processing of whole blood samples requires extra time, and could add 1-2 days to the turnaround time of the assay. | Top of Page | Bovine Viral Diarrhea - BVD Ear Notch Screen Pooled Testing | | Fee: 31.25 | Specimen: 1cm x 1cm ear tissue (1/2 inch notching pliers) | Shipping Container: 6 or 10 ml red top tube (no additive or gel) | Coolant: frozen gel packs | Days Tested: T,Th | Turnaround Time: 1-2 bus. days (see comments) | Comments: Please follow these Submission Guidelines. Samples will be run in pools up to 50. See Virology for BVD ear notch individual testing. Processing of ear notch samples requires extra time, and could add 1-2 days to the turnaround time of the assay. | Top of Page | Bovine Viral Diarrhea - BVD Genetic Analysis | | Fee: 149.00 | Specimen: 1) serum (at least 300 uL) 2) virus isolate (at least 300 uL) 3) tissue (1 cm3 or biopsy) | Shipping Container: 1,2) 1.5 ml microfuge tube 3) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested: weekly | Turnaround Time: 2-4 weeks | Comments: 2) Must come with a copy of a USDA shipping permit. 3) Shipping instruction: send next day delivery. | Top of Page | Circovirus Universal (Types 1 and 2) | | Fee: 44.50 | Specimen: 1) serum (at least 0.5 ml) 2) tissue 3) feces | Shipping Container: 1) leakproof 1.5 ml microfuge tube 2,3) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested: T,F | Turnaround Time: 1-2 bus. days (from test date) | | Comments: | Top of Page | Circovirus Type 2 | | Fee: 37.25 | Specimen: 1) semen (at least 0.5 ml) 2) serum (at least 0.5 ml) 3) tissue 4) blood swabs 5) feces 6) oral fluids/saliva (at least 2 ml) | Shipping Container: 1,4) leakproof 1.5 ml microfuge tube 2) red top tube 3,5) whirl-pac or plastic bag 6) sterile tube or whirl pac | Coolant: frozen gel packs | Days Tested: T,F | Turnaround Time: 1-2 bus. days (from test date) | | Comments: 6) Do not pool group/pen samples. | Top of Page | Circovirus Type 2 Genetic Analysis | | Fee: 121.75 | Specimen: 1) serum (at least 300 uL) 2) virus isolate (at least 300 uL) 3) tissue (1 cm3 or biopsy) 4) oral fluids/saliva (at least 2 ml) | Shipping Container: 1,2) 1.5 ml microfuge tube 3) whirl-pac or plastic bag 4) sterile tube or whirl pac | Coolant: frozen gel packs | Days Tested: weekly | Turnaround Time: 2-4 weeks | Comments: 2) Must come with a copy of a USDA shipping permit. 3) Shipping instruction: send next day delivery. 4) Do not pool group/pen samples. | Top of Page | Encephalomyocarditis Virus - EMC | | Fee: 32.50 | Specimen: 1) tissue 2) virus isolate | Shipping Container: 1) whirl-pac or plastic bag 2) leakproof 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested: biweekly (Th) | Turnaround Time: no more than 14 bus. days | | Comments: | Top of Page | Equine Encephalidites | | Fee: no charge | Specimen: brain tissue | Shipping Container: | Coolant: | Days Tested: daily | Turnaround Time: 4 bus. days | | Comments: Sent to Minnesota Dept. of Health. | Top of Page | Exotic Newcastle Disease Matrix (USDA) | | Fee: 39.50 | Specimen: tracheal swabs | Shipping Container: synthetic swabs | Coolant: frozen gel packs | Days Tested: W | Turnaround Time: 1-2 bus. days (from test date) | | Comments: Swabs may be pooled up to 5. | Top of Page | Exotic Newcastle Disease Fusion (USDA) | | Fee: 39.50 | Specimen: tracheal swabs | Shipping Container: synthetic swabs | Coolant: frozen gel packs | Days Tested: W | Turnaround Time: 1-2 bus. days (from test date) | | Comments: Swabs may be pooled up to 5. Fusion PCR is run to detect virulent NDV after a positive is obtained by END virus Matrix PCR testing. | Top of Page | Hemagglutinating Encephalomyelitis Virus - HEV (VWD) | | Fee: 32.50 | Specimen: 1) tissue 2) virus isolate 3) oral fluids | Shipping Container: 1) whirl-pac or plastic bag 2,3) leakproof 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested: M,Th | Turnaround Time: 5 bus. days | | Comments: | Top of Page
| Hemagglutinating Encephalomyelitis Virus (VWD) Full Length HE Gene Sequencing | | Fee: 149.00 | Specimen: 1) tissue 2) virus isolate 3) oral fluids | Shipping Container: 1) whirl-pac or plastic bag 2,3) leakproof 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested: as needed | Turnaround Time: 2-4 weeks | | Comments: |
Top of Page | Infectious Bovine Rhinotracheitis - IBR/BHV 1 | | Fee: 23.75 | Specimen: 1) swabs (nasal, conjunctival, vaginal or preputial) 2) lung or other tissue 3) virus isolate 4) tracheal wash or lavage | Shipping Container: 1) synthetic swabs 2) whirl-pac or plastic bag 3) leakproof 1.5 ml microfuge tube 4) sterile tube | Coolant: 1,2,4) frozen gel packs 3) dry ice | Days Tested: T | Turnaround Time: same day (as test date) | | Comments: Results reported on Tuesdays (samples must be recieved at least the day before test date). | Top of Page | Influenza Virus for Routine Testing - Group A Matrix RRT - PCR | | Fee: 30.00 | Specimen: 1) nasal or pharyngeal swabs 2) lung (2-3 cm3 or biopsy) 3) oral fluids/saliva (at least 2 ml) | Shipping Container: 1) synthetic swabs 2,3) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested: M,T,W,F | Turnaround Time: 2-3 bus. days | Comments: 1) For mammals 2) Shipping instruction: send next day delivery. 3) Do not pool group/pen samples. | Top of Page | Influenza Virus - Group A Matrix (USDA) | | Fee: 30.00 | Specimen: swabs | Shipping Container: synthetic swabs | Coolant: frozen gel packs | Days Tested: M,T,W,F | Turnaround Time: 2 bus. days | | Comments: For Avians. Swabs may be pooled up to 5. Results reported same day as test date. | Top of Page | Influenza Virus - Group A, Subtyping for H & N | | Fee: 22.00 | Specimen: 1) nasal or pharyngeal swabs 2) virus isolate (at least 0.5 ml) 3) lung (2-3 cm3 or biopsy) 4) oral fluids/saliva (at least 2 ml) | Shipping Container: 1) synthetic swabs 2) leakproof 1.5 ml microfuge tube 3) whirl-pac or plastic bag | Coolant: 1,3) frozen gel packs 2) dry ice | Days Tested: T,Th | Turnaround Time: 1-2 weeks | Comments: Subtyping tests are run on samples previously testing positive for influenza virus. 2) Must come with a copy of an USDA shipping permit. 3) Shipping instruction: send next day delivery. 4) Do not pool group/pen samples. | Top of Page | Influenza Virus - Group A, Subtyping for H5/H7 (USDA) | | Fee: 15.25 | Specimen: swabs | Shipping Container: synthetic swabs | Coolant: frozen gel packs | Days Tested: M,W | Turnaround Time: 24 hrs. (from test date) | | Comments: Testing for subtyping is run after a positive is obtained by Influenza virus Matrix testing. | Top of Page | Influenza Virus - USDA Differential N1 | | Fee: no charge | Specimen: 1) matrix influenza PCR positive sample 2) virus isolate | Shipping Container: 1) sterile tube 2) 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested: | Turnaround Time: | | Comments: Requires a written agreement with the USDA. | Top of Page | Influenza Virus Sequencing | | Fee: 149.00 | Specimen: 1) nasal swabs 2) viral isolate (at least 0.5 ml) 3) lung (2-3 cm3 or biopsy) 4) oral fluids/saliva (at least 2 ml) | Shipping Container: 1) synthetic swabs 2) leakproof 1.5 ml microfuge tube 3,4) whirl-pac or plastic bag | Coolant: 1,3,4) frozen gel packs 2) dry ice | Days Tested: weekly | Turnaround Time: 2-4 weeks | Comments: 2) Must come with a copy of an USDA shipping permit. 3) Shipping instruction: send next day delivery. 4) Do not pool group/pen samples. | Top of Page | Koi Herpes Virus | | Fee: 26.25 | Specimen: 1) tissue 2) culture media | Shipping Container: sterile container | Coolant: frozen gel packs | Days Tested: as needed | Turnaround Time: 3-5 bus. days | | Comments: | Top of Page | Porcine Cytomegalovirus - PCMV | | Fee: 62.00 | Specimen: 1) nasal turbinates 2) nasal swabs 3) buffy coat | Shipping Container: | Coolant: frozen gel packs | Days Tested: T | Turnaround Time: 1-2 weeks | | Comments: | Top of Page | Porcine Parvovirus - PPV | | Fee: ref. lab fee | Specimen: 1) fetal thoracic fluid 2) tissue | Shipping Container: 1) leakproof container 2) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested: | Turnaround Time: | | Comments: Sent to reference lab. | Top of Page | Porcine Reproductive and Respiratory Virus - NAEU PRRS, Life Tech | | Fee: 28.00 | Specimen: 1) semen (at least 1 ml) 2) serum (at least 0.5 ml) 3) tissue (1 cm3 or biopsy) 4) blood swabs 5) nasal swabs 6) oral fluids/saliva (at least 2 ml) | Shipping Container: 1,2,4) leakproof, 1.5 ml microfuge tube 3) whirl-pac or plastic bag 5) synthetic swabs 6) sterile tube or whirl pac | Coolant: frozen gel packs | Days Tested: M-F | Turnaround Time: same day (boar stud monitoring) or next bus. day (regular request) Holiday schedule for boar stud monitoring | Comments: 3) Shipping instruction: send next day delivery. 6) Do not pool group/pen samples. The Veterinary Diagnostic Laboratory prefers pooling samples at the lab. Additional information. | Top of Page | Porcine Reproductive and Respiratory Virus - NAEU Differential PRRS | | Fee: 28.00 | Specimen: 1) semen (at least 1 ml) 2) serum (at least 0.5 ml) 3) tissue (1 cm3 or biopsy) 4) blood swabs 5) nasal swabs 6) oral fluids/saliva (at least 2 ml) | Shipping Container: 1,2,4) leakproof, 1.5 ml microfuge tube 3) whirl-pac or plastic bag 5) synthetic swabs 6) sterile tube or whirl pac | Coolant: frozen gel packs | Days Tested: weekly | Turnaround Time: 1-7 bus. days | Comments: 3) Shipping instruction: send next day delivery. 6) Do not pool group/pen samples. The Veterinary Diagnostic Laboratory prefers pooling samples at the lab. Additional information. | Top of Page | Porcine Reproductive and Respiratory Virus - PRRS, Quantitative (U.S. Strain Only) | | Fee: 32.50 | Specimen: 1) semen (at least 1 ml) 2) serum (at least 0.5 ml) 3) tissue (1 cm3 or biopsy) | Shipping Container: 1,2) 1.5 ml microfuge tube 3) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested: T | Turnaround Time: 1-2 bus. days (from test date) | Comments: 3) Shipping instruction: send next day delivery. Additional information. | Top of Page | Porcine Reproductive and Respiratory Virus - PRRS, Genetic Analysis (Strain Differentiating/Sequencing) | | Fee: 135.25 | Specimen: 1) serum (at least 300 uL) 2) virus isolate (at least 300 uL) 3) tissue (1 cm3 or biopsy) 4) extracted RNA 5) oral fluids/saliva (at least 2 ml) | Shipping Container: 1,2) 1.5 ml microfuge tube 3) whirl-pac or plastic bag 4) sterile tube 5) sterile tube or whirl pac | Coolant: frozen gel packs | Days Tested: weekly | Turnaround Time: 2-4 weeks | Comments: 2) Must come with a copy of a USDA shipping permit. 3) Shipping instruction: send next day delivery. 5) Do not pool group/pen samples. Additional info. | Top of Page Porcine Reproductive and Respiratory Virus - PRRS, Tetracore EZ-PRRSV MPX 4.0 Real Time Multiplex | | Fee: 48.75 | Specimen: 1) semen (at least 1 ml) 2) serum (at least 0.5 ml) 3) tissue 4) blood swabs | Shipping Container: 1,2,4) leakproof, 1.5 ml microfuge tube 3) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested: as needed | Turnaround Time: 1 bus. day (from test day) | | Comments: 3) Shipping instruction: send next day delivery. Additional Information. | Top of Page | Porcine Rotavirus (Types A, B and C) | | Fee: 27.00 | Specimen: 1) feces 2) intestine 3) feedback material 4) environmental swabs 5) virus isolate | Shipping Container: 1,2,3) whirl-pac or leakpoof container 4) swab 5) 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested: M,W,F | Turnaround Time: 3 bus. days | | Comments: | Top of Page | Porcine Rotavirus A Sequencing (VP7 and VP4) | | Fee: 149.00 | Specimen: 1) PCR positive sample 2) virus isolate | Shipping Container: 2) 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested: M,W,F | Turnaround Time: 2-4 weeks | | Comments: | Top of Page | Porcine Rotavirus B Sequencing (VP7) | | Fee: 135.25 | Specimen: 1) PCR positive sample 2) Virus isolate | Shipping Container: 2) 1.5 ml microfuge tube | Coolant: Frozen gel packs | Days Tested: M,W,F | Turnaround Time: 2-4 weeks | | Comments: | Top of Page | Porcine Rotavirus C Sequencing (VP7) | | Fee: 135.25 | Specimen: 1) PCR positive sample 2) Virus isolate | Shipping Container: 2) 1.5 ml microfuge tube | Coolant: Frozen gel packs | Days Tested: M,W,F | Turnaround Time: 2-4 weeks | | Comments: | Top of Page | Spring Viremia of Carp | | Fee: 29.00 | Specimen: 1) tissue 2) culture media | Shipping Container: sterile container | Coolant: frozen gel packs | Days Tested: as needed | Turnaround Time: 3-5 bus. days | | Comments: | Top of Page | Swine Hepatitis E Virus | | Fee: 59.25 | Specimen: - Feces
- Fecal swabs
- Fresh small intestine
- Fresh liver tissue
| Shipping Container: - Whirl pac or plastic bag
- Synthetic swabs
- Leak proof container
- Leak proof container
| Coolant: Frozen gel packs, dry ice if already frozen | Days Tested: T | Turnaround Time: 1-2 weeks | | Comments: | Top of Page | Transmissible Gastroenteritis Virus - TGEV | | Fee: 31.25 | Specimen: 1) feces 2) fecal swab 3) fresh small intestine | Shipping Container: 1,3) whirl-pac or leakpoof container 2)swab | Coolant: frozen gel packs | Days Tested: M,W,F | Turnaround Time: 3 bus. days | | Comments: | Top of Page
| Transmissible Gastroenteritis Virus (TGEV) Full Length Spike Gene Sequencing | | Fee: 447.00 | Specimen: 1) feces 2) fecal Swab 3) fresh small intestine 4) viral isolate | Shipping Container: 1,3) whirl-pac or leakproof container 2) swab | Coolant: frozen gel packs | Days Tested: as needed | Turnaround Time: 2-4 weeks | | Comments: |
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| Transmissible Gastroenteritis Virus (TGEV) ORF3 Sequencing | | Fee: 135.25 | Specimen: 1) feces 2) fecal swab 3) fresh small intestine 4) viral isolate | Shipping Container: 1,3) whirl-pac or leakproof container 2) swab | Coolant: frozen gel packs | Days Tested: as needed | Turnaround Time: 2-4 weeks | | Comments: |
Top of Page Top of Page Note on days tested and turnaround times: Samples must be received at least 1 business day before the scheduled testing day. New client accounts can take up to 2-3 business days to establish before testing can begin. Samples may not always be tested on the first scheduled day after they arrive, but testing will usually be completed within the posted turnaround time. If rush testing is desired, please contact us. Top of Page
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